3T3-L1 Adipocyte Maintenance Medium, 500ml from Zen-Bio, Inc.

Product Specs
Item3T3-L1 Adipocyte Maintenance Medium, 500ml
CompanyZen-Bio, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAM-1-L1
Quantity500ml
Temperature4C
TypeMEDIA

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Zen-Bio, Inc.

3920 South Alston Ave

Durham, North Carolina 27713

United States

Phone: (866)234-7673 or (919)547-0692
(919)547-0693

Company Profile

Website: http://www.zen-bio.com

(1) Anti-Adipogenic Activity of Secondary Metabolites Isolated from Smilax sieboldii Miq. on 3T3-L1 AdipocytesInternational Journal of Molecular SciencesMay 16, 2023Yeon Woo Jung, Jung A Lee, Ji Eun Lee, Hanna Cha, Yun-Hyeok Choi, Wonsik Jeong, Chun Whan Choi, Joa Sub Oh, Eun-Kyung Ahn, Seong Su HongZenbio] was performed. The initiation medium was changed on day two with the 3T3-L1 Adipocyte Medium (AM-1-L1, Zenbio). On days four and six, the progression medium was replaced with the 3T3-L1 Adipocyte Medium

(2) Investigation on the Biological Safety and Activity of a Gluconolactone-Based Lotion for Dermocosmetic ApplicationPharmaceuticals (Basel)April 27, 2023Nicola Zerbinati, Serena Di Francesco, Maria Chiara Capillo, Cristina Maccario, Giorgio Stabile, Hassan Galadari, Raffaele Rauso, Sabrina Sommatis, Roberto Mocchi(DM-2-L1, Zen-Bio, Durham, NC, USA) for 3 days at 37 °C. Then, cells were maintained in maintenance medium (AM-1-L1, Zen-Bio, Durham, NC, USA) that was replaced every 2 days. Each cell line was grown in conditions of

(3) Development of a physiological insulin resistance model in human stem cell–derived adipocytesScience AdvancesJune 17, 2022Max Friesen, Andrew S. Khalil, M. Inmaculada Barrasa, Jacob F. Jeppesen, David J. Mooney, Rudolf Jaenischinstructions in 3T3-L1 Differentiation Medium (Zen-Bio DM-2-L1-500) and 3T3-L1 Adipocyte Medium (Zen-Bio AM-1-L1). TNFα treatment hPSC adipocytes were treated for 1 day at the end of the sensitization period with

(4) The middle lipin domain adopts a membrane-binding dimeric protein foldNature CommunicationsDecember 1, 2021Weijing Gu, Shujuan Gao, Huan Wang, Kaelin D. Fleming, Reece M. Hoffmann, Jong Won Yang, Nimi M. Patel, Yong Mi Choi, John E. Burke, Karen Reue, et al.differentiation medium (ZenBio, DM2L1500), and at day 2 were switched to maintenance medium (ZenBio, AM-1-L1). RNA isolation was performed at day 0, day 2, and day 4 of differentiation. Cell morphology was monitored

(5) Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexiaThe Journal of experimental medicineJune 7, 2021Rupert JE, Narasimhan A, Jengelley DHA, Jiang Y, Liu J, Au E, Silverman LM, Sandusky G, Bonetto A, Cao S, et al.incubator. Cells were cultured for 3 d in DM. Cells were then washed with PBS, adipocyte maintenance media (AM-1-L1; Zen Bio) was added to the wells, and the cells were placed back into the incubator. Cells were cultured

(6) GRIM19 Impedes Obesity by Regulating Inflammatory White Fat Browning and Promoting Th17/Treg BalanceCellsJanuary 15, 2021Jhun J, Woo JS, Lee SH, Jeong JH, Jung K, Hur W, Lee SY, Ryu JY, Moon YM, Jung YJ, et al.medium (DM2-L1, ZenBio) for 3 days, followed by incubation with 3T3-L1 adipocyte maintenance medium (AM-1-L1, ZenBio). For C2C12 cells, T3 and insulin were added to the medium at concentrations of 1 and 20 nM,

(7) Screening and Identification of an Anti-inflammatory and Anti-adipogenic Constituent from Ligularia taquetii NakaiNatural Product CommunicationsJanuary 1, 2020Sungchan Jang, Min-Seon Kim, Taejin Park, Ji H. Sim, Seung-Young Kimreplaced with the same concentrations of LTE diluted in 3T3-L1 Adipocyte Maintenance Medium (Cat. no. AM-1-L1, ZenBio, Research Triangle Park, United States). This maintenance medium (along with LTE) was replenished every

(8) Adipocyte-Derived Lipids Mediate Melanoma Progression via FATP ProteinsCancer DiscoveryAugust 1, 2018Maomao Zhang et al.cells were maintained in their adipocytic, differentiated state with maintenance media (ZenBio, Cat no. AM-1-L1). This adipocyte maintenance media (ZenBio, Cat no. AM-1-L1) was used for all coculture experiments. FATP1

(9) MicroRNA-30 modulates metabolic inflammation by regulating Notch signaling in adipose tissue macrophagesInternational journal of obesity (2005)June 1, 2018Miranda K, Yang X, Bam M, Murphy EA, Nagarkatti PS, Nagarkatti M.differentiation medium, and adipocyte maintenance medium were also used (Zen-Bio, PM-1-L1, DM-2-L1, AM-1-L1). Conditioned medium was collected between days 7 and 14 post-differentiation, 0.22um filtered, aliquoted,

(10) Role of Micro RNA-205 in Promoting Visceral Adiposity of NZ10 Mice with Polygenic Susceptibility for Type 2 DiabetesJournal of Diabetes & MetabolismDecember 15, 2015Nikhil Adi, Jennipher Adi, Liliana Cesar, Paul Kurlansky, Arthur Agatston, Keith A Webstermedium (DM-2-L1, Zen-Bio). After 3 days the DM-2-L1 media was replaced by adipocyte maintenance medium (AM-1-L1, Zen-Bio). Adipocytes were fed with AM-1-L1 every 3rd day. Preadipocytes were transfected with pre or

(11) Mechanisms of Peroxisome Proliferator Activated Receptor γ Regulation by Non-steroidal Anti-inflammatory DrugsNuclear Receptor SignalingOctober 5, 2015Ana C. Puhl et al.Rosiglitazone or NSAIDs for three days [Klemm et al., 2001]. Cells were then maintained in Zen Bio AM-1-L1 medium (Zen-Bio, Inc., Research Triangle Park, NC). On day 8, lipid accumulation in the adipocytes was

(12) Paracrine Interactions between Adipocytes and Tumor Cells Recruit and Modify Macrophages to the Mammary Tumor Microenvironment: The Role of Obesity and Inflammation in Breast Adipose TissueCancers (Basel)January 15, 2015Ana M. Santander, Omar Lopez-Ocejo, Olivia Casas, Thais Agostini, Lidia Sanchez, Eduardo Lamas-Basulto, Roberto Carrio, Margot P. Cleary, Ruben R. Gonzalez-Perez, Marta Torroella-Kouri(SVF) that pellets. Ex-vivo isolated adipocytes were then cultured in the ZenBio’s adipocyte medium AM-1-L1 (ZenBio Inc.), or they were mixed with macrophages and tumor cells in co-cultures as described below. 2.4.

(13) Macrophage Elastase Suppresses White Adipose Tissue Expansion with Cigarette SmokingAmerican Journal of Respiratory Cell and Molecular BiologyDecember 1, 2014Takao Tsuji, Neil J. Kelly, Saeko Takahashi, Adriana S. Leme, A McGarry McGarry Houghton, Steven D. Shapiro(Zen-Bio), moved to DM-2-L1 (Zen-Bio), and incubated for 3 days. Differentiated adipocytes were maintained in AM-1-L1 (Zen-Bio). After differentiation, 3T3-L1 adipocytes were incubated in DMEM plus 0.1% FBS with or without

(14) Capsaicin Induces “Brite” Phenotype in Differentiating 3T3-L1 PreadipocytesPLoS ONEJuly 29, 2014Ritesh K. Baboota, Dhirendra P. Singh, Siddhartha M. Sarma, Jaspreet Kaur, Rajat Sandhir, Ravneet K. Boparai, Kanthi K. Kondepudi, Mahendra Bishnoimedium (DMEM), 3T3-L1 adipocyte differentiation media (DM-2-L1) and 3T3-L1 adipocyte maintenance media (AM-1-L1) were obtained from Zenbio Inc. (Research Triangle Park, NC). Fetal calf serum (FCS), penicillin-streptomycin,

(15) Hkat, a novel nutritionally regulated transmembrane protein in adipose tissuesScientific ReportsDecember 21, 2012Ren ZhangZenbio, Research Triangle Park, NC), differentiation medium (DM-2-L1) and adipocyte maintenance medium (AM-1-L1). Briefly, 3T3 L1 cells was allowed to reach confluence in preadipocytes medium (day -2), and after

(16) Medium Chain Fatty Acids Are Selective Peroxisome Proliferator Activated Receptor (PPAR) γ Activators and Pan-PPAR Partial AgonistsPLoS ONEMay 23, 2012Marcelo Vizoná Liberato et al.Rosiglitasone, 1 mM HA C6, or 1 mM DA C10 as indicated in Figure legend. Cells were then fed with Zen Bio's AM-1-L1 media. On day 7 cells were fixed, stained with Red Oil O and phase contrast images were taken using

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3T3-L1 Adipocyte Maintenance Medium, 500ml from Zen-Bio, Inc.

Zen-Bio, Inc.

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3T3-L1 Adipocyte Maintenance Medium, 500ml from Zen-Bio, Inc.

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